Journal Title
Title of Journal: Biogerontology
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Abbravation: Biogerontology
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Publisher
Springer Netherlands
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Authors: R A Kireev A F Tresguerres E Vara C Ariznavarreta J A F Tresguerres
Publish Date: 2007/05/03
Volume: 8, Issue: 5, Pages: 469-482
Abstract
The aging theory postulates that this process may be due to the accumulation of oxidative damage in cells and molecules The present study has investigated the effect of castration in old female rats on various parameters related to the antioxidant properties of several cellular fractions obtained from the liver and the influence of several chronic treatments on it both in intact and castrated animals Sixtyone 22monthold Wistar female rats were used About 21 intact animals were divided into three groups and treated for 10 weeks with GH melatonin or saline and 40 ovariectomized at 12 months of age animals were divided into five groups and treated for the same time with GH melatonin estrogens Eos phytoestrogens Phyt or saline All animals were sacrificed at 24 months of age by decapitation The activity of glutathione peroxidase GPx in cytosolic fraction glutathioneStransferase GST in cytosol and microsomal fractions and the levels of Nitric oxide NO and cytochrome С in mitochondrial and cytosol fractions of liver were determined A decrease in GST activity was detected in cytosol and in the microsomal fraction in ovariectomized animals as compared to intact rats The activity of GPx was also decreased in ovariectomized as compared with the intact group NO level was increased and cytochrome С decreased in the mitochondrial fraction of the liver in ovariectomized females as compared with the intact group respectively No significant changes after melatonin or GH treatments were found in GPx GST activity and NO level in mitochondrial fraction in the intact group Administration of GH melatonin Eos and Phyt in the ovariectomized groups significantly increased the GPx and GST activity in the cytosol and microsomal fraction and decreased the level of NO in the mitochondrial fraction as compared with the untreated rats A significant increase in the level of cytochrome С in the mitochondrial fraction and a decrease in the cytosol fraction were also found with all treatments The administration of GH melatonin Eos and Phyt to castrated females seem to reduce oxidative changes in the liver from old ovariectomized rats
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