Authors: Sergio Murolo Gianfranco Romanazzi Adib Rowhani Angelantonio Minafra Pierfederico La Notte Maria Barbara Branzanti Vito Savino
Publish Date: 2007/09/01
Volume: 120, Issue: 2, Pages: 137-145
Abstract
Grapevine virus A GVA is considered one of the viruses associated with rugose wood RW one of the most economically important diseases of grapevine Thirtyseven GVA isolates collected from grapevine cultivars from Marche centraleastern Italy Apulia and Campania southern Italy were subjected to molecular characterization The genetic and population diversity was studied in the coat protein CP gene by RTPCRRFLP analysis with three restriction enzymes MseI AluI and AciI and nucleotide sequencing A new primer pair CP1F/R allowing amplification of the whole CP gene 621 bp was developed RFLP with AciI yielded the highest number of variants in GVA isolates showing seven different ‘simple’ profiles A B C D E F and G ‘Complex’ profiles were also found and the most common variant combination was A + B in 39 of isolates The analysis of GVA sequences confirmed the presence of plants infected with more than one GVA variant and suggested that RTPCRRFLP is suitable for evaluating population diversity of GVA enabling a screening of different haplotypes The distribution of RFLP profiles and the phylogenetic analysis were not correlated with the location of infected plants showing the presence of a GVA population with genetic diversity in the average with those of RNA virusesThe work was supported by the project “Characterization of grapevine viruses in Marche” funded by Marche Polytechnic University We are indebted to Drs Pasquale Saldarelli CNRIVV Bari Italy Maher Al Rwahnih and Cristina Rosa UC Davis Davis CA USA for advice on the interpretation of molecular data We are grateful to Prof GP Martelli for critical revision of the manuscript
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