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Title of Journal: Eur J Plant Pathol

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Abbravation: European Journal of Plant Pathology

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Springer Netherlands

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DOI

10.1016/0022-3697(63)90225-7

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ISSN

1573-8469

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Using monoclonal antibodies against the common epi

Authors: TsungChi Chen WeiTing Tsai YaChi Kang YunChi Wang ShyiDong Yeh
Publish Date: 2015/10/17
Volume: 144, Issue: 3, Pages: 509-524
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Abstract

Virus species in the genus Tospovirus can be classified into serogroups based on the serology of nucleocapsid protein one of the key criteria for the identification of a tospovirus Most globally important tospoviruses belong to the serogroups containing Tomato spotted wilt virus TSWV Watermelon silver mottle virus WSMoV and Iris yellow spot virus IYSV as type species In this investigation we attempted to develop a generic monoclonal antibody MAb against the common epitopes of the NSs proteins of TSWV and Impatiens necrotic spot virus INSV two representative tospoviruses prevalent in Europe and America The NSs proteins of TSWV and INSV were individually expressed in the bacterial pET system and the purified recombinant proteins denoted TNSs and INSs respectively were used to produce MAbs Results from indirect enzymelinked immunosorbent assays ELISAs and western blotting indicated that the selected MAbINSs55G4E7 against INSs broadly reacts with the NSs proteins of INSV Groundnut ringspot virus GRSV and Tomato chlorotic spot virus TCSV two members of the TSWV serogroup All obtained MAbs against TNSs are TSWV specific Our results indicate that the combination of MAbINSs55G4E7 and TSWVspecific MAbTNSs81D9E2 reacted with four tested EuroAmericatype tospoviruses Remarkably the threeinone combination of MAbINSs55G4E7 MAbTNSs81D9E2 and the previously prepared MAbWNSs which reacts with the common epitope of the NSs proteins of Asia type tospoviruses reacts with all 12 tested tospoviruses from different geographic regions Here we report an efficient serological tool for the prompt detection and differentiation of the most economically important tospoviruses prevalent in Europe America and AsiaThis study was supported by grants provided by the National Science Council NSC 992324B468003MY2 the Ministry of Education of Taiwan ROC under the ATU plan and the programme NSC 1012911I005301 of NCHUUCD Plant and Food Biotechnology Centre National Chung Hsing University

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