Authors: Ha Na Seo Bo Young Jeon Hung Thuan Tran Dae Hee Ahn Doo Hyun Park
Publish Date: 2008/05/31
Volume: 13, Issue: 2, Pages: 224-
Abstract
Saccharomyces sp SK0704 further defined as SK0704 isolated from longtermripening kimchi was identified by a biochemical method with an API kit its physiology was found to be very similar to that of S cerevisiae ATCC 26603 further defined as ATCC 26603 except in terms of starch utilization SK0704 did not excrete extracellular glucoamylase but utilized starch as a sole carbon source under only aerobic conditions Crude enzyme excreted from SK0704 catalyzed the saccharification of starch to glucose but ATCC 26603 did not The PCR product obtained using the chromosomal DNA of SK0704 and the primers designed on the basis of the extracellular glucoamylasecoding gene of S diastaticus was homologous with the intracellular sporulationspecific glucoamylase of S cerevisiae SDSPAGE pattern of soluble protein extracted from yeast cells grown on glucose was greatly different from that on starch From these results we proposed that the SK0704 may have a specific physiological function for starch catabolism such as membrane transport system and intracellular saccharification of starch
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