Authors: Yousef Amini Bagher Moradi Mohsen Tafaghodi Zahra Meshkat Kiarash Ghazvini Mahdi FasihiRamandi
Publish Date: 2016/11/11
Volume: 21, Issue: 5, Pages: 653-658
Abstract
Among various vaccine candidates subunit vaccines play an important role in immune protection against tuberculosis TB Calcium phosphate CP is considered as a strong inorganic adjuvant due to its great potential in increasing immune responses The purpose of this study was to evaluate specific immune responses following the administration of trifusionCP nanoparticles The physiochemical properties of these nanoparticles including morphology particle size zeta potential and adsorption rate were measured in vitro Subcutaneous immunization was performed three times on days 0 14 and 28 Two weeks after the last administration IFNgamma IL4 and TGFbeta levels were measured by indirect enzyme linked immunosorbent assay ELISA The trifusion protein was successfully adsorbed onto calcium phosphate nanoparticles The mean sizes of the resultant trifusion CPN and CPN were 9784 ± 1208 and 67 ± 1185 nm respectively CPN containing trifusion had stronger ability to induce IFNgamma than the control groups IL4 and TGFbeta secretions in trifusion and trifusionCPN groups were higher than those in the PBS group However there was no significant p 005 difference in IL4 and TGFbeta concentrations between trifusion group and trifusion CPN group Therefore calcium phosphate nanoparticles are good candidates for immunization against TB because antigen can be easily adsorbed onto CPN and strong cellular immune responses against CPNantigen can be stimulated
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