Authors: Alok Kumar Varshney Medicherla Krishna Mohan Ambarish Sharan Vidyarthi Vinod Kumar Nigam Purnendu Ghosh
Publish Date: 2014/01/04
Volume: 18, Issue: 6, Pages: 1176-1184
Abstract
The production of manganese peroxidase MnP by the whiterot fungus Phanerochaete chrysosporium NCIM 1197 was investigated by the screening and optimization of the media constituents and physiological factors MnP production by the fungus was used as the response to screen the media constituents with statistically valid PlackettBurman PB design Response surface methodology RSM was applied to optimize the level of screened media constituents Amongst the media constituents screened glucose maltose ammonium chloride and urea were selected as the most important for MnP enhancement A fivelevel Central Composite Design CCD was used in optimizing the important media constituents for maximizing the MnP production The optimal medium composition for maximum MnP production was 1388 mM of glucose 1388 mM of maltose 002 mM of ammonium chloride and 002 mM of urea The final experiment was conducted to validate the model which was shown to produce 7020 U/mL of MnP with a predicted value of 6649 U/mL on the 8th day of incubation
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