Authors: JaeHo Lee SeungJun Lee KyungBaeg Roh EunSun Jung DeokHoon Park
Publish Date: 2015/03/18
Volume: 20, Issue: 1, Pages: 194-200
Abstract
Threedimensional 3D spheroid culture has recently become a valuable tool to evaluate the utility of treatments that better mirror biological features and exhibit high concordance with in vivo conditions Here by using the 3D spheroid method we investigated cell morphology proliferation melanin content tyrosinase activity and cAMP production During 3D speroid cultures melanin biosynthesis was observed and the amount of melanin continuously increased until cell proliferation slowed down Upregulation of the cAMP pathway enhanced tyrosinase activity allowing melanin synthesis However after treatment with depigmenting agents melanin content was reduced These results indicate that 3D spheroid culture method is an important tool to study melanin biosynthesis which enables us to overcome several limitations found in the 2D adherent cell culture mehtod
Keywords: