Authors: Jiyoung Nam Min Young Jung Pyoung Il Kim Hyang Burm Lee Si Wouk Kim Chul Won Lee
Publish Date: 2015/09/25
Volume: 20, Issue: 4, Pages: 708-713
Abstract
Cyclic lipopeptides were produced by Bacillus amyloliquefaciens subsp plantarum strain BC321 that was isolated from yellow loess soil in the Jeonnam province of South Korea Several lipopeptides were isolated from the bacteria using organic solvent extraction and reversephase highperformance liquid chromatography RPHPLC Purified iturin surfactin and fengycintype lipopeptides were identified using liquid chromatographymass spectrometry LCMS analysis Among the lipopeptides C17fengycin B showed strong antifungal activity against the phytopathogenic fungus Fusarium oxysporum f sp radicislycopersici and then the fengycin was further characterized by UV Fourier transforminfrared spectroscopy FTIR and LCMS/MS analyses C17fengycin B was highly produced at quantities of up to 15 µg/mL at 37°C whereas little amount of the fengycin was produced at 25°C Purified C17fengycin B inhibited mycelial growth of F oxysporum with a minimal inhibitory concentration of 50 µg/mL This study suggests that C17fengycin B is a major antifungal component produced by the BC321 strain that could be used as an environmentally friendly agent to control the phytopathogenic F oxysporum
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