Molecular characterization of ORFs 2 to 7 of Korea

Authors: Hyun Na Koo Jeong Mi Oh Jae Kyung Lee Jae Young Choi Kwang Sik Lee Jong Yul Roh Yeon Ho Je Byung Rae Jin Sung Sik Yoo Jae Su Kim Young In Kim In Joong Yoon Soo Dong Woo

Publish Date: 2008/12/24

Volume: 46, Issue: 6, Pages: 709-719

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Abstract

To determine the characteristics of the Korean porcine reproductive and respiratory syndrome virus PRRSV CA which was isolated from the serum of an infected pig in 2006 we investigated the nucleotide sequence and expression of the structural ORFs ORFs 2 to 7 using the bApGOZA system We found that the structural ORFs 2 to 7 of CA consisted of 3188 nucleotides that were the same as those formed from VR2332 Comparison of the CA with the other strains revealed nucleotide sequence identity ranging from 898 to 995 To better understand the genetic relationships between other strains phylogenetic analyses were performed The CA strain was closely related to the other North American genotype strains but formed a distinct branch with high bootstrap support Additionally expression levels of the PRRSV proteins in insect cells were strong or partially weak The results of this study have implications for both the taxonomy of PRRSV and vaccine development

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