Emphasis Type="Italic"Halomonas jeotgali/Emphas

Authors: MinSoo Kim Seong Woon Roh JinWoo Bae

Publish Date: 2010/06/23

Volume: 48, Issue: 3, Pages: 404-410

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Abstract

A moderate halophilic Gramnegative nonmotile rodshape and aerobe designated as strain HwaT was isolated from traditional fermented Korean seafood which presented as a single cell or paired cells Optimal growth occurred at 25°C in 10 w/v salts at pH 70–80 however growth occurred in a temperature range of 10–32°C a salts concentration of 5–25 w/v and pH 50–100 Tests for oxidase and catalase were positive The cells produced polyβhydroxybutyric acid but not exopolysaccharide Based on the 16S rRNA gene sequence not only was there low similarity between strain HwaT and all other species 941 similarity with H subglaciescola DSM 4683T 940 similarity with H sulfidaeris Esulfide1T 936 similarity with H cerina SP4T and 930 similarity with H halodurans DSM 5160T but the phylogenetic analysis revealed that the isolate may be classified as a novel species belonging to the genus Halomonas in the class Gammaproteobacteria The predominant fatty acids of strain HwaT were C181 ω7c C160 C120 3OH and C161 ω7c/C150 iso 2OH The DNA G+C content was calculated as 617 mol Based on phenotypic genotypic and phylogenetic characteristics it is proposed that the strain designated as HwaT be assigned to the genus Halomonas as Halomonas jeotgali sp nov =KCTC 22487T =JCM 15645T

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