Monitoring nutrient impact on bacterial community

Authors: Myungsu Kim Seung Seob Bae Mijin Seol JungHyun Lee YoungSook Oh

Publish Date: 2008/12/24

Volume: 46, Issue: 6, Pages: 615-

PDF Link

Abstract

Marine harbor sediments are frequently polluted with significant amount of polycyclic aromatic hydrocarbons PAHs some of which are naturally toxic recalcitrant mutagenic and carcinogenic To stimulate biodegradation of PAHs in PAHcontaminated sediments collected from near Gwangyang Bay Korea lactate was chosen as a supplementary carbonaceous substrate Sediment packed into 600 ml airtight jar was either under no treatment condition or lactate amended condition 1 w/v Microbial community composition was monitored by bacteriaspecific and archaeaspecific PCRterminal restriction fragment length polymorphism TRFLP in addition to measuring the residual PAH concentration Results showed that lactate amendment enhanced biodegradation rate of PAHs in the sediment by 4 to 8 times and caused a significant shift in archaebacterial community in terms of structure and diversity with time Phylogenetic analysis of 23 archaeal clones with distinctive RFLP patterns among 288 archaeal clones indicated that majority of the archaeal members were closest to unculturable environmental rDNA clones from hydrocarboncontaminated and/or methanogenesisbearing sediments Lactate amendment led to the enrichment of some clones that were most closely related to PAHdegrading Methanosarcina species These results suggest a possible contribution of methanogenic community to PAH degradation and give us more insights on how to effectively remediate PAHcontaminated sediments

Keywords: