KSHV infection of Bcell lymphoma using a modified

Authors: Hyosun Cho Hyojeung Kang

Publish Date: 2012/04/27

Volume: 50, Issue: 2, Pages: 285-292

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Abstract

Kaposi’s sarcomaassociated herpesvirus KSHV is the causative agent of two B cell lymphoproliferative diseases namely primary effusion lymphoma PEL and multicentric Castleman’s disease MCD KSHV infection of B cell lymphoma in vitro has been a longstanding battle in advancing human KSHV biology In this study a modified form of KSHV BAC36 named BAC36A significantly increased the fidelity of genetargeted sitedirected mutagenesis in the KSHV genome This modification eliminates tedious screening steps required to obtain mutant clones when a KSHV BAC36 reverse genetic system is used Coculturing Bcell lymphoma BJAB cells with KSHV BAC36A stably transfected 293T cells enabled us to infect BJAB cells with a KSHV virion derived from the KSHV BAC36A The coculture system produced substantial amounts of KSHV infection to BJAB meaning that KSHV virions were released from 293T cells and then infected neighboring BJAB cells Owing to our success with the KSHV BAC36A and coculture system we propose a new genetic system for the study of KSHV gene expression and regulation in Bcell lymphoma

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