Molecular cloning and characterization of Emphasi

Authors: Lan Ji Huang Jinghua Cui Hong Hua Piao Yeongjin Hong Hyon E Choy Phil Youl Ryu

Publish Date: 2010/11/03

Volume: 48, Issue: 5, Pages: 663-667

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Abstract

Cytolysin A ClyA is a poreforming hemolytic protein encoded by the clyA gene It has been identified in Salmonella enterica serovars Typhi and Paratyphi A To identify and characterize the clyA genes in various Salmonella enterica strains 21 different serotypes of strains isolated from clinical specimens were presently examined Fulllength clyA genes were found in S enterica serovar Brandenburg Indiana Panama and Schwarzengrund strains by polymerase chain reaction amplification The ClyA proteins from these four strains showed 97 amino acid identity to that of S enterica serovar Typhi Although all four serovars expressed detectable levels of ClyA as determined by Western blot analysis they did not show a strong hemolytic effect on blood agar indicating that ClyA may not be efficiently expressed or secreted Escherichia coli transformed with clyA genes from the four serovars enhanced production of ClyA proteins and hemolytic activities to a level similar to S enterica serovar Typhi ClyA The present results suggest that ClyA may play a role in the pathogenesis of S enterica serovar Brandenburg Indiana Panama and Schwarzengrund

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