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Title of Journal: Vet Res Commun

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Abbravation: Veterinary Research Communications

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Springer Netherlands

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DOI

10.1002/jsfa.2138

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1573-7446

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ISG15 expression in response to doublestranded RN

Authors: C Liu R Chang X Yao W T Qiao Y Q Geng
Publish Date: 2009/04/15
Volume: 33, Issue: 7, Pages: 723-733
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Abstract

Fetal bovine lung FBL cells are used in the culture of viruses which infect cattle and ISG15 plays a role in innate immunity against viral infections However whether the expression of ISG15 gene can be induced in FBL cells is still unknown In this work the expression of ISG15 in cultured FBL cells was detected after stimulated with poly IC or LPS Realtime PCR analyses revealed that the transcript of ISG15 can be induced by poly IC or LPS The increased expression of free ISG15 was confirmed via Western blotting Furthermore immunofluorescence assays demonstrated that IRF3 was translocated from the cytoplasm to the nucleus in the FBL cells treated with poly IC Chromatin immunoprecipitation assays showed that IRF3 can bind to the promoter of the bISG15 gene To demonstrate IRF3 can promote the expression of bISG15 we establish a luciferasereporter system of bovine ISG15 gene in 293 T cells The luciferase assay showed that the overexpression of bovine IRF3 could activate the promoter of bISG15 gene Taken together these results suggest that the expression of bISG15 can be induced in FBL cells stimulated with poly IC or LPS and IRF3 may play a role in inducing the expression of ISG15 in FBL cells

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