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Title of Journal: J Plant Biochem Biotechnol

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Abbravation: Journal of Plant Biochemistry and Biotechnology

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Springer India

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DOI

10.1007/bfb0041135

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0974-1275

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Assessment of Genetic Diversity and Identification

Authors: D Nagaich A Chandra
Publish Date: 2009/06/18
Volume: 18, Issue: 2, Pages: 257-260
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Abstract

Caribbean stylo Stylosanthes hamata is a tropical fodder and cover crop Along with four other Stylosanthes species S scabra S humilis S viscosa S guianensis it was introduced in India It became well adapted in certain parts of the country and has been recommended for the improvement of range and degraded lands A collection of 63 S hamata accessions was fingerprinted with RAPID ISSR and STS markers Though the mean discriminating power of these marker systems ranges from 065 to 071 high values of marker index 291 resolving power 1492 and effective number of patterns per assay unit 5065 makes ISSR as a better marker system in comparison to other two markers used in this study Thirteen RAPD and eleven STS primers could differentiate a maximum of 42 and 17 accessions respectively whereas two ISSR primers produced distinct fingerprints of all the S hamata accessions Mean genetic similarities of accession ranged from 083 ISSR to 091 RAPD Two RAPD two STS and four ISSR primers generated a set of 12 diagnostic markers which could be useful for germplasm characterization and management


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