Salinity mediated biochemical changes towards diff

Authors: Nirjhar Dasgupta Parmita Nandy Chandan Sengupta Sauren Das

Publish Date: 2012/12/16

Volume: 23, Issue: 1, Pages: 31-41

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Abstract

Cellular ion homeostasis through osmotic adjustment and efficient free radical scavenging ability are considered as fundamental salt tolerance determinants of plants Increased salinity ~30 ppt in Indian Sundarbans has incurred detrimental impact on vegetation pattern and leading to the precarious existence of some taxa A comparative account on some physiological and biochemical determinants towards salt stress were assayed in order to focus on the extent of adaptability among the three true mangroves An in vitro experiment was carried out with mangrove seedlings of Avicennia marina Bruguiera gymnorrhiza and Heritiera fomes in differential NaCl treatments Among the five sets of treatment 0 100 200 300 and 400 mM leaf osmotic potential ψ shows consistent hike in A marina and B gymnorrhiza along the salinity gradient at least up to 300 mM 77 and 58  respectively over control and comparatively poor in 400 mM On the other hand H fomes reaches its maximum OP value in 200 mM NaCl treatment 64  increment from control Thin layer Chromatography was employed for assessing seven free amino acids and spectrophotometric quantification of them from leaf tissues grown in different salinity gradients were performed The two antioxidative enzymes Peroxidase and Superoxide dismutase were estimated qualitatively and quantitatively in three taxa at different salinity level As compared to other two taxa H fomes depicted fewer numbers of isoforms of two enzymes in PAGE analysis and less enzyme activity across the salinity gradients Considering the studied parameters H fomes might be endorsed towards the feeble adaptability in the present day’s elevated salinity of Indian Sundarbans

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