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Title of Journal: J Plant Biochem Biotechnol

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Abbravation: Journal of Plant Biochemistry and Biotechnology

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Springer India

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DOI

10.1016/j.biochi.2006.07.003

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0974-1275

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Expression of biologically active antithrombosis

Authors: Chunfeng Guan Xilong Du Gang Wang Jing Ji Chao Jin Xiaozhou Li
Publish Date: 2013/04/05
Volume: 23, Issue: 3, Pages: 257-265
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Abstract

Lumbrokinase LK is an important fibrinolytic enzyme derived from earthworm The capsules of the extracts of LK have been widely used Unfortunately the life cycle of earthworm is long and extraction of lumbrokinases is generally a labor intensive and timeconsuming activity Also the extract is easily contaminated by multiple components The successful expression of the recombinant LK provides a way to obtain single component with fibrinolytic activity Meanwhile it was reported that LK could be orally administered Therefore we have been attempting to produce recombinant LK using a safe and costeffective production system In this stduy LK gene placed under the seedspecific promoter napA was expressed in the Helianthus annuus L The SDSPAGE and Western blot analysis confirmed the expression of recombinant LK rLK The yield of rLK was about 51 g/kg sunflower seeds as determined by ELISA Fibrin plate assays revealed that the crude extraction of rLK from sunflower seed kernel contained a high level of fibrinolytic activity Following oral administration of T1 generation of transgenic sunflower seed kernel the prothrombin time PT the activated partial thromboplastin time APTT the thrombin time TT and the carrageenininduced thrombosis were evaluated using Balb/c mice as the thrombosis model It was demonstrated that the oral treatment of mice with transgenic sunflower seed kernel had a significant antithrombotic effect The finding provides a way for lowcost and seed kernel edible delivery of human therapeutic proteinsThis work was supported by the National Genetically Modified Organism Major Projects of China Breeding of Transformed Maize with Higher Nutrient Absorption Efficiency 2011ZX08003005 National Natural Science Foundation of China 31271793 and 2010 PhD supervisor Doctoral Program of Higher Specialized Research Fund 20100032110060

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  2. Plant Hsp90 and Its Partner Proteins
  3. Assessment of Genetic Diversity and Identification of Informative Molecular Markers for Germplasm Characterization in Caribbean Stylo ( Stylosanthes hamata )
  4. Integration of hup Genes into the Genome of Chickpea- Rhizobium Through Site-Specific Recombination
  5. Structure, Regulation and Biosynthesis of Phosphoenolpyruvate Carboxylase from C 4 Plants
  6. Microsatellite marker-based diversity and population genetic analysis of selected lowland and mid-altitude maize landrace accessions of India
  7. Effect of Hormones on Tumor Formation in Tobacco Hybrids
  8. Unraveling the nutritional and therapeutic properties of ‘Kavuni’ a traditional rice variety of Tamil Nadu
  9. DNase and RNase Responses of Petunia × hybrida During Transition to Flowering as Affected by Light Treatments
  10. Molecular cloning and sequence comparison of a cDNA encoding α-glucan, water dikinase (GWD) from potato ( Solanum tuberosum L.), and analysis of gene expression
  11. pUC-IVT, a modified pUC19 based in vitro transcription vector
  12. Higher amount of steviol detected in the leaves of a non-toxic endangered medicinal herb, Aconitum heterophyllum
  13. Micropropagation of Anethum graveolens L Through Axillary Shoot Proliferation
  14. Salinity mediated biochemical changes towards differential adaptability of three mangroves from Indian Sundarbans
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