Authors: R K Sharma A K Wakhlu M Boleria
Publish Date: 2012/12/30
Volume: 13, Issue: 2, Pages: 157-159
Abstract
A protocol is described for rapid and largescale in vitro propagation of Anethum graveolens by enhanced axillary shoot induction that was dependent on BAP supply The synergistic combination of 05 mg l−1 BAP and 01 mg l−1 IBA induced 100 shoot formation as well as shoot number 66 ± 048 per explant Subculturing of shoot tips of in vitro plants on multiplication medium enabled continuous production of healthy shoots with similar frequency Rooting of shoots was achieved on a medium with 1mg l−1 IBA and 05 mg l−1 Kn Micropropagated plants established in garden were uniform and identical to the donor plant with respect to morphological and cytological characteristics
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