Microsatellite markerbased diversity and populati

Authors: Samanthi K Wasala B M Prasanna

Publish Date: 2012/11/06

Volume: 22, Issue: 4, Pages: 392-400

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Abstract

Maize Zea mays L harbours significant genetic diversity not only in its centre of origin Mexico but also in several countries worldwide including India in the form of landraces In this study DNA fingerprinting of 48 landrace accessions from diverse regions of India was undertaken using 42 fluorescent dyelabeled Simple Sequence Repeat SSR markers followed by allele resolution using DNA sequencer and analysis of molecular diversity within and among these landraces The study revealed a large number of alleles 550 with high mean number of alleles per locus 131 and Polymorphism Information Content PIC of 060 reflecting the level of diversity in the landrace accessions Besides identification of 174 unique alleles in 44 accessions six highly frequent SSR alleles were detected at six loci phi014 phi090 phi112 umc1367 phi062 and umc1266 with individual frequencies greater than 075 indicating that chromosomal regions harboring these SSR alleles are not selectively neutral F statistics revealed very high genetic differentiation population subdivision and varying levels of inbreeding in the landraces Analysis of Molecular Variance showed that 63  of the total variation in the accessions could be attributed to withinpopulation diversity and 37  represented between population diversity Cluster analysis of SSR data using Nei’s genetic distance and UPGMA revealed considerable genetic diversity in these populations although no clear separation of accessions was observed based on their geographic originThe study was undertaken as a part of the ICAR National Fellow Project 2005–2010 awarded to BMP The authors thank the Division of Germplasm Conservation NBPGR New Delhi especially Dr Kalyani Srinivasan for providing seed material of some landrace accessions used in this study

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