An efficient callus proliferation protocol and rha

Authors: Junli Wang Yuan Lu Qian Wang Kun Liu Yunfei Song Kaili Bi

Publish Date: 2011/04/27

Volume: 20, Issue: 2, Pages: 252-257

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Abstract

An efficient callus proliferation system for Rheum franzenbachii Munt a rare medicinal plant has been developed Callus induced from leaf explants incubated on Murashige and Skoog MS medium with appropriate supplements of plant growth regulators In the 6benzylaminopurine 6BAP in combination with αnaphthalene acetic acid NAA treatments different concentrations of NAA showed different induction effects on explants When concentration of 6BAP was as high as 20 mgl−1 in combination with 05 mgl−1 NAA the callus induction rate reached 583 NphenylN’123thiadiazol5ylure TDZ in combination with NAA was very suitable for callus proliferation compared to TDZ in combination with 24dicholorophenoxy acetic acid 24D or TDZ in combination with indole3acetic acid IAA Fresh and dry weight of callus cultured on MS medium supplemented with 05 mgl−1 TDZ in combination with 02 mgl−1 NAA increased 263 and 150 times within 35 days culture respectively Quantitative analysis of rhaponticin by HPLC showed that the phytochemical profile of callus was similar to that of wild plants and the content of rhaponticin in callus cultured on MS medium supplemented with 05 mgl−1 TDZ and 02 mgl−1 NAA was 166 mgg−1DW compared to that of 40 mgg−1 DW in wild plantsThis work was financially supported by National Basic Research Program of China 973 Program 2009CB522300 the Fund of State Key Laboratory of Phytochemistry and Plant Resources in West China 09708211Z1 Program of State Ethnic Affairs Commission 09ZY09 the “985” Project MUC985 “111” Project B08044 and the Fundamental Research Funds for the Central Universities 0910KYZY46

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