Authors: A V Romanenko S E Shepelev
Publish Date: 2009/02/04
Volume: 40, Issue: 4, Pages: 270-278
Abstract
Using a microelectrode technique we studied the effects of alimentary vitamin В1 deficiency on synaptic transmission in isolated phrenicohemidiaphragmatic murine preparations Animals of group І control were on a standard thiaminecontrolled diet 16 mg/kg thiamine with no limitations Animals of group II control with alimentary limitation were on the same diet but daily consumption in these animals was limited and made similar to the amount of food consumed by the animals of group ІІІ within idential periods of cage housing for differentiation of the effects of anorexia related to the thiaminedeficient state in group III and proper effects of В1 hypovitaminosis Animals of group ІІІ thiaminedeficient were on a standard diet with no limitations mostly analogous to that in group І but containing no thiamine In phrenicohemidiaphragmatic preparations obtained from animals of group ІІІ the amplitude of endplate potentials EPPs and miniature EPPs mEPPs on the 10th day of consumption of the thiaminedefficient diet and the quantum composition of EPPs on the 20th day became significantly Р 001 smaller than in preparations obtained from animals of both groups І and ІІ The frequency of mEPPs and membrane potential of muscle fibers in group ІІІ remained unchanged Two processes a decrease in the dimension of the transmitter quantum which is observed within rather early stages of the development of thiaminedefficient state and a decrease in the quantum composition of evoked EPPs at later stages underlie a gradual decrease in the amplitude of EPPs related to the development of alimentary vitamin В1 deficiency
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