Authors: L M Sokurenko Yu B Chaikovskii
Publish Date: 2016/08/30
Volume: 48, Issue: 3, Pages: 171-175
Abstract
We studied the toxic action of mercury II chloride 109 μM on cultured IMR32 neuroblastoma cells and possible protective effects of the antioxidant thiotriazolinum and cardioprotector mildronate concentrations of the former agent in the medium 001100 mg/ml Isolated addition of thiotriazolinum and a combination of thiotriazolinum + mildronate to the medium did not cause dramatic negative effects number of dead cells at all concentrations used did not exceed 7–10 of the population under control conditions Thiotriazolinum in concentrations of 01 and 001 mg/ml demonstrated a significant neuroprotective activity against the toxic action of mercury II chloride the mean number of living cells under such conditions of culturing was on average about 830 vs 547 in the case where isolated action of HgCl2 was used The mean number of living morphologically unaffected cells cultured in the medium containing 109 μM HgCl2 with the addition of mildronate + thiotriazolinum reached 870 of the control value taken as 100 Therefore under the toxic action of HgCl2 on cultured IMR32 neuroblastoma cells the combination of mildronate and thiotriazolinum demonstrated a significant protective effect which was more intense than that of thiotriazolinum at its isolated application Thiotriazolinum in concentrations of 0001 to 01 mg/ml exerts rather moderate negative effects on the studied culture of neuroblastoma cells
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