Authors: Ling Xiao Changming Lu Bing Zhang Huijie Bo Yuhua Wu Gang Wu Yinglong Cao Deyue Yu
Publish Date: 2009/04/09
Volume: 18, Issue: 5, Pages: 733-746
Abstract
Gene transferability from transgenic rapeseed to various subspecies and varieties of Brassica rapa was assessed in this study Artificial crossability was studied in 118 cultivars of 7 B rapa subspecies and varieties with the transgenic rapeseed GT73 Brassica napus as the pollen donor On average 57 seeds were obtained per pollination with a range from 005 to 194 The heading type of B rapa L showed significantly higher crossability than nonheading types of B rapa The spontaneous outcrossing rate between B rapa female and the transgenic rapeseed Ms8 × Rf3 B napus male ranged from 0039 to 0406 with an average of 019 The fertilization process and the development of the hybrid seeds as shown by fluorescent staining techniques indicated that the number of adhered pollens on the stigma was reduced by 80 the number of pollen tubes in the style was reduced by 2/3 and the fertilization time was delayed by over 20 h when pollinated with the transgenic rapeseed Ms8 × Rf3 in comparison with the bud selfpollination of B rapa as control About 10–70 of the interspecific hybrid embryos were aborted in the course of development Some seeds looked cracked in mature pods which showed germination abilities lower than 10 The spontaneous outcrossing rates were much lower than the artificial crossability and their survival fitness of the interspecific hybrid was very low indicating that it should be possible to keep the adventitious presence of the offplants under the allowed threshold if proper measures are takenThis study was supported by the Major Special Project for Development of Transgenic Organisms 2008–2010 Ministry of Agriculture China and the Social Welfare Project on Environmental Protection Ministry of Environmental Protection China 2009–2011
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