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Title of Journal: Transgenic Res

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Abbravation: Transgenic Research

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Springer Netherlands

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DOI

10.1016/0145-2134(79)90128-5

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1573-9368

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Expression of the human granulocyte–macrophage col

Authors: I A Burkov I A Serova N R Battulin A V Smirnov I V Babkin L E Andreeva G A Dvoryanchikov O L Serov
Publish Date: 2013/02/23
Volume: 22, Issue: 5, Pages: 949-964
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Abstract

Expression of the human granulocyte–macrophage colonystimulating factor hGMCSF gene under the control of the 5′regulatory sequence of the goat alphaS1casein gene with and without a matrix attachment region MAR element from the Drosophila histone 1 gene was studied in four and eight transgenic mouse lines respectively Of the four transgenic lines carrying the transgene without MAR three had correct tissuesspecific expression of the hGMCSF gene in the mammary gland only and no signs of cell mosaicism The concentration of hGMCSF in the milk of transgenic females varied from 19 to 14 μg/ml One line presented hGMCSF in the blood serum indicating ectopic expression The values of secretion of hGMCSF in milk of 6 transgenic lines carrying the transgene with MAR varied from 005 to 07 μg/ml and two of these did not express hGMCSF Three of the four examined animals from lines of this group showed ectopic expression of the hGMCSF gene as determined by RTPCR and immunofluorescence analyses as well as the presence of hGMCSF in the blood serum Mosaic expression of the hGMCSF gene in mammary epithelial cells was specific to all examined transgenic mice carrying the transgene with MAR but was never observed in the transgenic mice without MAR The mosaic expression was not dependent on transgene copy number Thus the expected “protective or enhancer effect” from the MAR element on the hGMCSF gene expression was not observedThe authors express gratitude to Dr Nelly Khaidarova Institute of Molecular Genetics Academy of Sciences of Russia Moscow Russia for help in preparation of recombinant DNA and Dr Irina Gileva for providing us the recombinant human GMCSF derived from E coli This study was financially supported by Grant5626 from Program of Fundamental Research RAN


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  2. Modification of gibberellin signalling (metabolism & signal transduction) in sugar beet: analysis of potential targets for crop improvement
  3. Phenotyping soybean plants transformed with rd29A:AtDREB1A for drought tolerance in the greenhouse and field
  4. Impact of Bt -corn MON88017 in comparison to three conventional lines on Trigonotylus caelestialium (Kirkaldy) (Heteroptera: Miridae) field densities
  5. Assessment of gene flow from a herbicide-resistant indica rice ( Oryza sativa L.) to the Costa Rican weedy rice ( Oryza sativa ) in Tropical America: factors affecting hybridization rates and characterization of F 1 hybrids
  6. Fibroblast growth factor (Fgf) signaling pathway regulates liver homeostasis in zebrafish
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  8. Development and characterization of transgenic mouse models for conditional gene knockout in the blood–brain and blood-CSF barriers
  9. Transgenic tobacco simultaneously overexpressing glyphosate N -acetyltransferase and 5-enolpyruvylshikimate-3-phosphate synthase are more resistant to glyphosate than those containing one gene
  10. Gene transferability from transgenic Brassica napus L. to various subspecies and varieties of Brassica rapa
  11. EFSA’s scientific activities and achievements on the risk assessment of genetically modified organisms (GMOs) during its first decade of existence: looking back and ahead
  12. Proteome rebalancing in transgenic Camelina occurs within the enlarged proteome induced by β-carotene accumulation and storage protein suppression
  13. Transportable data from non-target arthropod field studies for the environmental risk assessment of genetically modified maize expressing an insecticidal double-stranded RNA
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  15. Understanding plant defence responses against herbivore attacks: an essential first step towards the development of sustainable resistance against pests

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