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Title of Journal: Transgenic Res

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Abbravation: Transgenic Research

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Springer International Publishing

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DOI

10.1002/app.40683

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1573-9368

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Proteome rebalancing in transgenic Emphasis Type=

Authors: Monica A Schmidt Ken Pendarvis
Publish Date: 2016/10/22
Volume: 26, Issue: 2, Pages: 171-186
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Abstract

Oilseed crops are global commodities for their oil and protein seed content We have engineered the oilseed Camelina sativa to exhibit increased protein content with a slight decrease in oil content The introduction of a phytoene synthase gene with an RNAi cassette directed to suppress the storage protein 2S albumin resulted in seeds with an 11–24  elevation in overall protein The phytoene synthase cassette alone produced enhanced βcarotene content of an average 275 ± 610 μg/g dry seed and an overall altered seed composition of 11  less protein and comparable nontransgenic amounts of both oil and carbohydrates Stacking an RNAi to suppress the major 2S storage protein resulted in seeds that contain elevated protein and slight decrease in oil and carbohydrate amounts showing that Camelina rebalances its proteome within an enlarged protein content genotype In both βcarotene enhanced seeds with/without RNAi2S suppression the seed size was noticeably enlarged compared to nontransgenic counterpart seeds Metabolic analysis of maturing seeds indicate that the enhanced βcarotene trait had the larger effect than the RNAi2S suppression on the seed metabolome The use of a GRAS generally regarded as safe βcarotene as a visual marker in a floral dip transformation system such as Camelina might eliminate the need for costly regulatory and controversial antibiotic resistance markers βcarotene enhanced RNAi2S suppressed Camelina seeds could be further developed as a rapid heterologous protein production platform in a nonfood crop leveraging its enlarged protein content and visual markerThe authors are grateful to UC Davis Proteomics Core Facility for conducting the amino acid composition analysis and to Metabolon for metabolite analysis We thank Howard Berg for photography assistance Carbohydrate analysis was performed by the Complex Carbohydrate Research Center University of Georgia supported by the Chemical Sciences Geosciences and Biosciences Division Office of Basic Energy Sciences US Department of Energy Grant DEFG0293ER20097 to Parastoo Azadi at the Complex Carbohydrate Research Center


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Other Papers In This Journal:

  1. Transgenic production of arachidonic acid in oilseeds
  2. Expression of the human granulocyte–macrophage colony stimulating factor ( hGM - CSF ) gene under control of the 5′-regulatory sequence of the goat alpha-S1-casein gene with and without a MAR element in transgenic mice
  3. Modification of gibberellin signalling (metabolism & signal transduction) in sugar beet: analysis of potential targets for crop improvement
  4. Phenotyping soybean plants transformed with rd29A:AtDREB1A for drought tolerance in the greenhouse and field
  5. Impact of Bt -corn MON88017 in comparison to three conventional lines on Trigonotylus caelestialium (Kirkaldy) (Heteroptera: Miridae) field densities
  6. Assessment of gene flow from a herbicide-resistant indica rice ( Oryza sativa L.) to the Costa Rican weedy rice ( Oryza sativa ) in Tropical America: factors affecting hybridization rates and characterization of F 1 hybrids
  7. Fibroblast growth factor (Fgf) signaling pathway regulates liver homeostasis in zebrafish
  8. Chimeric analysis of EGFP and DsRed2 transgenic mice demonstrates polyclonal maintenance of pancreatic acini
  9. Development and characterization of transgenic mouse models for conditional gene knockout in the blood–brain and blood-CSF barriers
  10. Transgenic tobacco simultaneously overexpressing glyphosate N -acetyltransferase and 5-enolpyruvylshikimate-3-phosphate synthase are more resistant to glyphosate than those containing one gene
  11. Gene transferability from transgenic Brassica napus L. to various subspecies and varieties of Brassica rapa
  12. EFSA’s scientific activities and achievements on the risk assessment of genetically modified organisms (GMOs) during its first decade of existence: looking back and ahead
  13. Transportable data from non-target arthropod field studies for the environmental risk assessment of genetically modified maize expressing an insecticidal double-stranded RNA
  14. Genetically engineered Thompson Seedless grapevine plants designed for fungal tolerance: selection and characterization of the best performing individuals in a field trial
  15. Understanding plant defence responses against herbivore attacks: an essential first step towards the development of sustainable resistance against pests

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