Paper Search Console

Home Search Page About Contact

Journal Title

Title of Journal: Transgenic Res

Search In Journal Title:

Abbravation: Transgenic Research

Search In Journal Abbravation:

Publisher

Springer International Publishing

Search In Publisher:

DOI

10.1007/bf00307841

Search In DOI:

ISSN

1573-9368

Search In ISSN:
Search In Title Of Papers:

Transgenic tobacco simultaneously overexpressing g

Authors: Yunjun Liu Gaoyi Cao Rongrong Chen Shengxue Zhang Yuan Ren Wei Lu Jianhua Wang Guoying Wang
Publish Date: 2015/03/31
Volume: 24, Issue: 4, Pages: 753-763
PDF Link

Abstract

5Enolpyruvylshikimate3phosphate synthase EPSPS and glyphosate Nacetyltransferase GAT can detoxify glyphosate by alleviating the suppression of shikimate pathway In this study we obtained transgenic tobacco plants overexpressing AM79 aroA GAT and both of them respectively to evaluate whether overexpression of both genes could confer transgenic plants with higher glyphosate resistance The transgenic plants harboring GAT or AM79 aroA respectively showed good glyphosate resistance As expected the hybrid plants containing both GAT and AM79 aroA exhibited improved glyphosate resistance than the transgenic plants overexpressing only a single gene When grown on media with high concentration of glyphosate seedlings containing a single gene were severely inhibited whereas plants expressing both genes were affected less When transgenic plants grown in the greenhouse were sprayed with glyphosate less damage was observed for the plants containing both genes Metabolomics analysis showed that transgenic plants containing two genes could maintain the metabolism balance better than those containing one gene after glyphosate treatment Glyphosate treatment did not lead to a huge increase of shikimate contents of tobacco leaves in transgenic plants overexpressing two genes whereas significant increase of shikimate contents in transgenic plants containing only a single gene was observed These results demonstrated that pyramiding both aroA and GAT in transgenic plants can enhance glyphosate resistance and this strategy can be used for the development of transgenic glyphosateresistant cropsThis work was funded by the National Major Project for Transgenic Organism Breeding 2013ZX08003001 We gratefully thank the anonymous reviewers for their valuable suggestions We acknowledge the technical staffs from Shanghai Sensichip Infotech Co Ltd for metabolic profiling We also give our thanks to Dr Liang Li from Biotechnology Research Institute of CAAS for insightful suggestion


Keywords:

References


.
Search In Abstract Of Papers:
Other Papers In This Journal:

  1. Transgenic production of arachidonic acid in oilseeds
  2. Expression of the human granulocyte–macrophage colony stimulating factor ( hGM - CSF ) gene under control of the 5′-regulatory sequence of the goat alpha-S1-casein gene with and without a MAR element in transgenic mice
  3. Modification of gibberellin signalling (metabolism & signal transduction) in sugar beet: analysis of potential targets for crop improvement
  4. Phenotyping soybean plants transformed with rd29A:AtDREB1A for drought tolerance in the greenhouse and field
  5. Impact of Bt -corn MON88017 in comparison to three conventional lines on Trigonotylus caelestialium (Kirkaldy) (Heteroptera: Miridae) field densities
  6. Assessment of gene flow from a herbicide-resistant indica rice ( Oryza sativa L.) to the Costa Rican weedy rice ( Oryza sativa ) in Tropical America: factors affecting hybridization rates and characterization of F 1 hybrids
  7. Fibroblast growth factor (Fgf) signaling pathway regulates liver homeostasis in zebrafish
  8. Chimeric analysis of EGFP and DsRed2 transgenic mice demonstrates polyclonal maintenance of pancreatic acini
  9. Development and characterization of transgenic mouse models for conditional gene knockout in the blood–brain and blood-CSF barriers
  10. Gene transferability from transgenic Brassica napus L. to various subspecies and varieties of Brassica rapa
  11. EFSA’s scientific activities and achievements on the risk assessment of genetically modified organisms (GMOs) during its first decade of existence: looking back and ahead
  12. Proteome rebalancing in transgenic Camelina occurs within the enlarged proteome induced by β-carotene accumulation and storage protein suppression
  13. Transportable data from non-target arthropod field studies for the environmental risk assessment of genetically modified maize expressing an insecticidal double-stranded RNA
  14. Genetically engineered Thompson Seedless grapevine plants designed for fungal tolerance: selection and characterization of the best performing individuals in a field trial
  15. Understanding plant defence responses against herbivore attacks: an essential first step towards the development of sustainable resistance against pests

Search Result: