Authors: Yunjun Liu Gaoyi Cao Rongrong Chen Shengxue Zhang Yuan Ren Wei Lu Jianhua Wang Guoying Wang
Publish Date: 2015/03/31
Volume: 24, Issue: 4, Pages: 753-763
Abstract
5Enolpyruvylshikimate3phosphate synthase EPSPS and glyphosate Nacetyltransferase GAT can detoxify glyphosate by alleviating the suppression of shikimate pathway In this study we obtained transgenic tobacco plants overexpressing AM79 aroA GAT and both of them respectively to evaluate whether overexpression of both genes could confer transgenic plants with higher glyphosate resistance The transgenic plants harboring GAT or AM79 aroA respectively showed good glyphosate resistance As expected the hybrid plants containing both GAT and AM79 aroA exhibited improved glyphosate resistance than the transgenic plants overexpressing only a single gene When grown on media with high concentration of glyphosate seedlings containing a single gene were severely inhibited whereas plants expressing both genes were affected less When transgenic plants grown in the greenhouse were sprayed with glyphosate less damage was observed for the plants containing both genes Metabolomics analysis showed that transgenic plants containing two genes could maintain the metabolism balance better than those containing one gene after glyphosate treatment Glyphosate treatment did not lead to a huge increase of shikimate contents of tobacco leaves in transgenic plants overexpressing two genes whereas significant increase of shikimate contents in transgenic plants containing only a single gene was observed These results demonstrated that pyramiding both aroA and GAT in transgenic plants can enhance glyphosate resistance and this strategy can be used for the development of transgenic glyphosateresistant cropsThis work was funded by the National Major Project for Transgenic Organism Breeding 2013ZX08003001 We gratefully thank the anonymous reviewers for their valuable suggestions We acknowledge the technical staffs from Shanghai Sensichip Infotech Co Ltd for metabolic profiling We also give our thanks to Dr Liang Li from Biotechnology Research Institute of CAAS for insightful suggestion
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