Paper Search Console

Home Search Page About Contact

Journal Title

Title of Journal: Transgenic Res

Search In Journal Title:

Abbravation: Transgenic Research

Search In Journal Abbravation:

Publisher

Springer Netherlands

Search In Publisher:

ISSN

1573-9368

Search In ISSN:
Search In Title Of Papers:

Development and characterization of transgenic mou

Authors: Matthew H Crouthamel Edward J Kelly Rodney J Y Ho
Publish Date: 2011/05/03
Volume: 21, Issue: 1, Pages: 113-130
PDF Link

Abstract

For many CNS acting drugs penetration into the central nervous system CNS is limited by the bloodCNSbarriers In an effort to quantitate the role of the protein components that make up the bloodCNSbarriers we created transgenic mice that allow conditional gene knockout using Cre/loxP technology We targeted the expression of Crerecombinase to the choroid plexus the bloodcerebral spinal fluid barrier using the lymphotropic papovavirus control region LPVcr and to brain endothelium the blood–brainbarrier using the proximal promoter region of the human von Willebrand Factor gene hVWFf We verified that LPVcr restricts expression to the choroid plexus in adult mice by using the LPVcr to drive nLacZ expression in transgenic mice The LPVCre and hVWFCre plasmids were then constructed and tested for Crerecombinase function in vitro and subsequently used to create transgenic mice The resulting transgenic mice were characterized for celltype specific Cremediated endonuclease activity by crossing them with transgenic mice containing a loxPflankedLacZ/EGFP dual reporter gene Z/EG The dual CreZ/EG transgenic offspring were evaluated for the location of EGFP mRNA expression by reverse transcriptase PCR and for protein expression by immunohistochemistry Immunohistochemistry for EGFP verified expression in the target cells and no ectopic expression outside of the expected cell types The LPVCre0607 transgenic line expressed functional Cre only in the choroid plexus and hVWFCre1304 line in brain endotheliumWe thank Terry Kavanagh for the use of the Cryocut cryomicrotome Richard Palmiter for the pLacF plasmid Nancy Jahroudi for the pHGHK plasmid Terry Van Dyke for the pLST plasmid Corrinne Lobe for the Z/EG plasmid Washington University ES Cell Core for the pTurboCre plasmid and Kerstin Verdina for assistance in editing This work was supported by the National Institute of Health grants NS39178 AI077390 and AI52663 Rodney J Y Ho is also supported by the Milo Gibaldi Endowment


Keywords:

References


.
Search In Abstract Of Papers:
Other Papers In This Journal:

  1. Transgenic production of arachidonic acid in oilseeds
  2. Expression of the human granulocyte–macrophage colony stimulating factor ( hGM - CSF ) gene under control of the 5′-regulatory sequence of the goat alpha-S1-casein gene with and without a MAR element in transgenic mice
  3. Modification of gibberellin signalling (metabolism & signal transduction) in sugar beet: analysis of potential targets for crop improvement
  4. Phenotyping soybean plants transformed with rd29A:AtDREB1A for drought tolerance in the greenhouse and field
  5. Impact of Bt -corn MON88017 in comparison to three conventional lines on Trigonotylus caelestialium (Kirkaldy) (Heteroptera: Miridae) field densities
  6. Assessment of gene flow from a herbicide-resistant indica rice ( Oryza sativa L.) to the Costa Rican weedy rice ( Oryza sativa ) in Tropical America: factors affecting hybridization rates and characterization of F 1 hybrids
  7. Fibroblast growth factor (Fgf) signaling pathway regulates liver homeostasis in zebrafish
  8. Chimeric analysis of EGFP and DsRed2 transgenic mice demonstrates polyclonal maintenance of pancreatic acini
  9. Transgenic tobacco simultaneously overexpressing glyphosate N -acetyltransferase and 5-enolpyruvylshikimate-3-phosphate synthase are more resistant to glyphosate than those containing one gene
  10. Gene transferability from transgenic Brassica napus L. to various subspecies and varieties of Brassica rapa
  11. EFSA’s scientific activities and achievements on the risk assessment of genetically modified organisms (GMOs) during its first decade of existence: looking back and ahead
  12. Proteome rebalancing in transgenic Camelina occurs within the enlarged proteome induced by β-carotene accumulation and storage protein suppression
  13. Transportable data from non-target arthropod field studies for the environmental risk assessment of genetically modified maize expressing an insecticidal double-stranded RNA
  14. Genetically engineered Thompson Seedless grapevine plants designed for fungal tolerance: selection and characterization of the best performing individuals in a field trial
  15. Understanding plant defence responses against herbivore attacks: an essential first step towards the development of sustainable resistance against pests

Search Result: