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Title of Journal: Transgenic Res

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Abbravation: Transgenic Research

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Springer Netherlands

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DOI

10.1007/bf02800965

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ISSN

1573-9368

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Transgenic production of arachidonic acid in oilse

Authors: James R Petrie Pushkar Shrestha Srinivas Belide Maged P Mansour Qing Liu James Horne Peter D Nichols Surinder P Singh
Publish Date: 2011/05/01
Volume: 21, Issue: 1, Pages: 139-147
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Abstract

We describe a transgenic microalgal Δ9elongase pathway transformed in both Brassica napus and Arabidopsis thaliana seed resulting in the production of arachidonic acid ARA This pathway is noteworthy for both the production of ARA in seed tissue and the low levels of intermediate C20 fatty acids that accumulate We also demonstrate that the arachidonic acid is naturally enriched at the sn2 position in triacylglycerol This is the first report of ARA production by the Δ9elongase pathway in an oilseedWe thank Anne Mackenzie for binary construct cloning Dion Frampton for cloning the I galbana gene Sue Blackburn Stan Robert and other members of the CSIRO microalgal research group for their ongoing advice and support Dan Holdsworth for management of the GC–MS facility and Lijun Tian and Adam White for their excellent technical assistance

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  10. Gene transferability from transgenic Brassica napus L. to various subspecies and varieties of Brassica rapa
  11. EFSA’s scientific activities and achievements on the risk assessment of genetically modified organisms (GMOs) during its first decade of existence: looking back and ahead
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