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Title of Journal: Biometals

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Abbravation: BioMetals

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Springer Netherlands

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DOI

10.1002/humu.22344

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1572-8773

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A role for ferritin in the antioxidant system in c

Authors: Alexandra Bottcher Paula Macedo Nobile Paula Fabiane Martins Fábio Frangiotti Conte Ricardo Antunes Azevedo Paulo Mazzafera
Publish Date: 2010/11/03
Volume: 24, Issue: 2, Pages: 225-237
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Abstract

Iron Fe is an essential nutrient for plants but it can generate oxidative stress at high concentrations In this study Coffea arabica L cell suspension cultures were exposed to excess Fe 60 and 240 μM to investigate changes in the gene expression of ferritin and antioxidant enzymes Iron content accumulated during cell growth and Western blot analysis showed an increase of ferritin in cells treated with Fe The expression of two ferritin genes retrieved from the Brazilian coffee EST database was studied CaFER1 but not CaFER2 transcripts were induced by Fe exposure Phylogenetic analysis revealed that CaFER1 is not similar to CaFER2 or to any ferritin that has been characterised in detail The increase in ferritin gene expression was accompanied by an increase in the activity of antioxidant enzymes Superoxide dismutase guaiacol peroxidase catalase and glutathione reductase activities increased in cells grown in the presence of excess Fe especially at 60 μM while the activity of glutathione Stransferase decreased These data suggest that Fe induces oxidative stress in coffee cell suspension cultures and that ferritin participates in the antioxidant system to protect cells against oxidative damage Thus cellular Fe concentrations must be finely regulated to avoid cellular damage most likely caused by increased oxidative stress induced by Fe However transcriptional analyses indicate that ferritin genes are differentially controlled as only CaFER1 expression was responsive to Fe treatmentThe authors are grateful to Dr Janette Palma Fett Universidade Federal do Rio Grande do Sul for providing the polyclonal antiferritin antibody used in this study This work was supported by grants from Fundação de Amparo a Pesquisa do Estado de São Paulo Fapesp and Conselho Nacional de Desenvolvimento Científico e Tecnológico Brasil CNPq

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  2. In vitro heme and non-heme iron capture from hemoglobin, myoglobin and ferritin by bovine lactoferrin and implications for suppression of reactive oxygen species in vivo
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