Production of CCHF viruslike particle by a baculo

Authors: Zhaorui Zhou Manli Wang Fei Deng Tianxian Li Zhihong Hu Hualin Wang

Publish Date: 2011/10/07

Volume: 26, Issue: 5, Pages: 338-

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Abstract

CrimeanCongo Haemorrhagic Fever Virus CCHFV is a tickborn virus of the Nairovirus genus within the Bunyaviridae family which is widespread and causes high fatality The nucleocapsid of CCHFV is comprised of N proteins that are encoded by the S segment In this research the N protein of CCHFV was expressed in insect cells using a recombinant baculovirus Under an electron microscope VirusLike Particles VLPs with various size and morphology were observed in cytoplasmic vesicles in the infected cells Sucrosegradient purification of the cell lysate indicated that the VLPs were mainly located in the upper fraction after ultracentrifugation which was confirmed by Western blot analysis and immunoelectron microscopy IEMFoundation items The Knowledge Innovation Program of the Chinese Academy of Sciences Grant No KSCX2YWN065 the Knowledge Innovation Program of the Chinese Academy of Sciences Grant No KSCX2EWG8 National Key Basic Research Program 973 Program Grant No 2010CB530103 National Basic Research Priorities Program of China Grant No 2007 FY210700

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