Establishment and preliminary application of a rap

Authors: Pengcheng Yu Xinjun Lv Xinxin Shen Qing Tang Guodong Liang

Publish Date: 2013/07/25

Volume: 28, Issue: 4, Pages: 223-227

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Abstract

The World Health Organization WHO standard assay for determining levels of the rabies virus neutralization antibody RVNA is the rapid fluorescent focus inhibition test RFFIT which is used to evaluate the immunity effect after vaccination against rabies For RFFIT CVS11 was used as the challenge virus BSR cells as the adapted cells and WHO rabies immunoglobulin WHO STD as the reference serum in this study With reference to WHO and Pasteur RFFIT procedures a microRFFIT procedure adapted to our laboratory was produced and its specificity and reproducibility were tested We tested levels of RVNA in human serum samples after immunization with different human rabies vaccines domestic purified Vero cell rabies vaccine PVRV and imported purified chick embryo cell vaccine PCECV using different regimens Zagreb regimen and Essen regimen We analyzed the levels of RVNA and compared the immune efficacy of domestic PVRV and imported PCECV using different immunization regimens The results showed that the immune efficacy of domestic PVRV using the Zagreb regimen was as good as that of the imported PCECV but virus antibodies were generated more rapidly with the Zagreb regimen than with the Essen regimen The RFFIT procedure established in our laboratory will enhance the comprehensive detection ability of institutions involved in rabies surveillance in China

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