Journal Title
Title of Journal: Virol Sin
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Abbravation: Virologica Sinica
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Publisher
Springer Singapore
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Authors: Yuting Qiao Zhiwei Sui Guoliang Hu Huabin Cao Guoxiang Yang Yong Li Yongsong Lei Lihua Zhao Quanjiao Chen
Publish Date: 2016/08/10
Volume: 31, Issue: 4, Pages: 331-338
Abstract
Hepatitis A virus is a pathogen associated with water pollution Contaminated drinking water can cause hepatitis A outbreaks lead to economic losses and even threaten human lives It is difficult to detect low levels of hepatitis A virus in water so the virus must be concentrated in order to quantify it accurately Here we present a simple rapid efficient technique for the concentration and detection of hepatitis A virus in water Our data showed that adding phosphatebuffered saline to the water prefiltering the water and adding Trizol reagent directly to the filtration membrane can significantly improve concentration efficiency Of three types of filtration membranes studied mixed cellulose ester membrane polyvinylidene fluoride membrane and nylon membrane the concentration efficiency using mixed cellulose ester membrane with a 01μm pore size was the highest reaching 9262 ± 517 This method was used to concentrate hepatitis A virus in water samples from Donghu Lake Using SYBR Green realtime reverse transcription polymerase chain reaction analysis the detection sensitivity of this method reached 101 copies/μL and its concentration efficiency reached 7945 ± 988
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