Authors: James L Pipkin W G Hinson R J Feuers L E LynCook E R Burns P H Duffy R Hart D A Casciano
Publish Date: 2013/07/02
Volume: 6, Issue: 2, Pages: 121-131
Abstract
A single intraperitoneal injection of the human therapeutic drug bleomycin BL was administered to three groups of male Fischer 344 rats at time 0 and the incorporation of 35Smethionine “synthesis” and phosphorylation patterns of stress proteins sps/hsps from bone marrow cells were analyzed over time by two dimensional electrophoresis and fluorography Two groups of rats young ad libitum Y/AL — 3 months and old ad libitum O/AL — 28 months had free access to rat chow and a third group of old rats O/CR — 28 months were maintained on a caloric restricted intake 60 of the AL diet The administration of BL in Y/AL O/AL and O/CR animals activated the 35S labeling of sp 90 which reached a peak at 4 hours Labeling of sp 90 was significantly greater in Y/AL compared to O/AL and the incorporation pattern of O/CR was intermediate to Y/AL and O/AL animals All labeling of sp 90 in each group had disappeared by 10 hours after BL administration Stress protein 70x inducible form in these three animal groups displayed a similar pattern of 35Sincorporation but the amount of labeling was less than that of sp 90 No labeling of sp 70x remained by 13 hours after BL administration Phosphorylation 32P phosphate incorporation of sp 90 reached a maximum level at 2 hours in all animals and 32P labeling in Y/AL was significantly increased over O/AL and O/CR with an intermediate level found in O/CR animals The turnover rate phosphorylation/dephosphorylation of sp 90 induced by BL was significantly suppressed and temporarily extended in O/AL as compared with O/CR which implied that CR not only increased incorporation of sp 90 but also enhanced a utilization of the phosphate pool very similar to that seen in Y/AL animals Aging Clin Exp Res 6 121 131 1994
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