Distribution and Speciation of Cu in the Root Bord

Authors: Cheng Peng Yi Wang Lijuan Sun Chen Xu Lijuan Zhang Jiyan Shi

Publish Date: 2015/12/17

Volume: 96, Issue: 3, Pages: 408-414

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Abstract

Root border cells RBCs serve plants in their initial line of defense against stress from the presence of heavy metals in the soil In this research light microscopy and synchrotronbased scanning transmission Xray microscopy STXM combined with near edge Xray absorption fine structure spectroscopy NEXAFS with a nanoscale spatial resolution were used to investigate the effects of copper Cu upon the RBCs as well as its distribution and speciation within the RBCs of rice Oryza sativa L under aeroponic culture The results indicated that with increasing exposure time and concentration the attached RBCs were surrounded by a thick mucilage layer which changed in form from an ellipse into a strip in response to Cu ion stress Copper was present as CuII which accumulated not only in the cell wall but also in the cytoplasm To our knowledge this is the first time that STXM has been used in combination with NEXAFS to provide new insight into the distribution and speciation of metal elements in isolated plant cellsThe work was supported by the National Natural Science Foundation of China 21177109 11179025 41422107 and Program for New Century Excellent Talents in University NCET110455 We would like to express our gratitude to Xiangjun Zhen and Zhi Guo at beamline BL08U1A of the Shanghai Synchrotron Radiation Facility for their generous help in data collection and processing We are also grateful to Chidong Zhou in Zhejiang University for his help in the art processing

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