Authors: Thammasit Vongsetskul Piyamaporn Phurayar Threeraphat Chutimasakul Patoomratana Tuchinda Sukanda Uamsiri Supeecha Kumkate Phorutai Pearngam Jirasak Jitpibull Chonchanok Samphaongern Pramuan Tangboriboonrat
Publish Date: 2016/04/19
Volume: 73, Issue: 12, Pages: 3319-3331
Abstract
Acanthus ebracteatus Vahl also known as sea holly is a traditional Thai herb used to treat skin diseases To treat the diseases the patients are required to take a bath with water boiled with the whole sea holly plants regularly This makes travel inconvenient for the patients To solve this problem a controlledrelease system for sea holly extract is required In this work the extract of A ebracteatus Vahl 5 w/v was loaded into electrospun cellulose acetate CA 12 w/v MW ~3 × 104 g/mol fibers 460 ± 84 nm The homogenous fibers were obtained using an applied voltage and fiber collection distance of 125 kV and 10 cm respectively Fourier transform infrared spectroscopy thermogravimetric analysis and differential scanning calorimetry revealed that the extract was uniformly dispersed in the CA domains ~99 of the loaded extract in the fibers was released in the acetate buffer at 37 °C via a diffusion mechanism while only ~35 of it could be done from the corresponding cast films The 22diphenyl1picrylhydrazyl DPPH· scavenging assay confirmed an antioxidant activity of the loaded fibers The incorporation of the extract into the fibers could greatly reduce the cytotoxicity of the extract and the MTT assay at 37 °C indicated ~52 of L929 mouse fibroblast cell viabilityThis work was supported by the Thailand Research Fund TRF/Mahidol University under Grant TRG5680070 Thailand Ministry of Science and Technology and Mahidol University Faculty of Science and TRF/Commission on Higher Education under Grant RTA5480007
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