Hepatitis C and B virus infection in Chinese patie

Authors: Mingyi Chen Zhiqiang Huang Lezhen Chen Yabing Gao Ruiyun Peng Dewen Wang

Publish Date: 2002/03/01

Volume: 1, Issue: 1, Pages: 21-27

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Abstract

In China the incidence of extrahepatic bile duct carcinoma EBDC tends to increase over the past decades The etiology of the noted increase in EBDC is not identified Approximately in a half of the overall Chinese patients with EBDC the causative factors in the development of EBDC have not been demonstrated There is a high prevalence of hepatitis C virus HCV or hepatitis B virus HBV in China both of which can induce malignant transformation of infected cells and strongly associated with hepatocellular carcinoma HCC In this study EBDC tissues from Chinese patients were examined for the presence of HCV and HBV infection to investigate further the potential causes of EBDCHCV NS5 protein and HBsAg were detected by labeled streptavidin biotin LSAB method HCV RNA and HBV DNA were detected by in situ polymerase chain reaction ISPCR in formalin fixed paraffin embedded specimens from 51 Chinese patients with EBDC HCV RNA and HBV DNA were detected by ISPCR in 34 Chinese patients with specimens of benign lesions of hepatobiliary tract control groupIn 51 case tissue sections of EBDC NS5 protein was detected in 14 275 and HBsAg in 5 98 HCV RNA in 18 354 and HBV DNA in 8 159 respectively of which HCV and HBV coinfection was detected in 2 39 In 34 case tissue sections of the control group HCV RNA was detected in 2 59 and HBV DNA in 3 88In this study using standard histochemical and PCR techniques HCV and HBV genomes and their encoding proteins were detected in the tissues of EBDC The data show that there is a higher than expected incidence of HCV and HBV presence in EBDC tissues than would be expected on serologic grounds The detectable rate of HCV RNA in EBDC tissues was significantly higher than in control group χ2=9808P=0002 As a result this study indicates that there is a correlation between the presence of HCV infection and EBDC and HCV infection has possible etiologic significance in the development of EBDC in China While HBV DNA was detected in EBDC tissues with the difference in the detectable rate of HBV DNA being not significance between EBDC tissues and the control group χ2=0853P=0356 Further research is necessary to determine the presence of a causal relationship between HCV/HBV infection and the development of EBDC

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