Expression of PEPT2 mRNA in the lung of rat with b

Authors: Li Li Dianhua Wang Xuan Zhang Xing Song

Publish Date: 2013/10/17

Volume: 12, Issue: 10, Pages: 468-472

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Abstract

Pulmonary fibrosis is a common pathological phenomena in lung cancer patients after chemotherapy or radiotherapy which is a key factor hindering to transport ion of high concentrated drug to the lung tissue peptide transporter has become targets of the rational design of peptides and peptide drug The purpose of the study is to investigate the expression of PEPT2 mRNA in the lung of rats with bleomycin BLMinduced pulmonary fibrosisFifty healthy adult SpragneDawley rats were randomized into five groups the rats in BLM 7d 14d and 28d groups were treated with a single instillation of 5 mg/kg of BLM to induced pulmonary fibrosis models On days 7 14 and 28 the animals were killed by exsanguination respectively Normal saline NS group were treated by NS on days 14 the animals were killed by exsanguinations Control group were untreated The lung samples were processed for light microscopy and determined the hydroxyproline HYP concentration The expression of PEPT2 mRNA were measured by RTPCR PEPT2 cDNA fragments were tested by dideoxy chain terminationCompared with control and NS group HYP levels increased on day 7 of BLM group but there was no statistical significant difference P 005 HYP levels markedly increased on days 14 and 28 of BLM group there was statistical significant difference P 001 The morphological study showed that collagenous fiber proliferated on days 14 and 28 of BLM group especially on day 28 formed pulmonary fibrosis There were no significant changes of pulmonary PEPT2 mRNA expression at different groups P 005

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