Survivin ASODN targeted therapy in XWLC05 cell tr

Authors: Weiwei Wang Shaojia Wang Gaofeng Li Lei Li Ruibing Cheng

Publish Date: 2012/06/08

Volume: 11, Issue: 6, Pages: 324-326

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Abstract

We established XWLC05 transplanted nude mice model 44 mice would be divided randomly into 4 groups control group blank Lip group simple liposome survivin SODN group transfected by sense oligonucleotide and survivin ASODN group transfected by antisense oligonucleotide We would study general activities of nude mice in these 4 groups measure the size of tumor and calculate the tumor inhabiting rate also Pathological methods were applied in the analysis of the effect of different treatment on heart kidney and liver of nude mice in these 4 groupsTumor grew slowly and size weight of tumor was lower in survivin ASODN group when compared with that of others Nude mice of survivin ASODN group showed lower growth index and tumor inhabiting rate was significantly higher than that of other groups P 005 Transplanted tumor on nude mice in control group blank Lip group and survivin SODN group grew bigger as time passed and there was no significance among them P 005 We found a great deal of tumor cell necrosis in survivin ASODN group No death of nude mice was observed in all 4 groups and we did not found obvious lesion in vital organsSupported by grants from the Yunnan Provincial Scientific Office Scientific Development Plan Basic Research of Social Development No 2009ZC120M Key project of Social Development of Yunnan Provincial No 2010CA015 United Fund of Science and Technology Bureau of Yunnan Province No 2010CD185

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