Authors: Liguo Zhao Yan Zhu Wantong Niu Meining Li Niuliang Cheng
Publish Date: 2011/04/19
Volume: 10, Issue: 4, Pages: 220-224
Abstract
The Sp1 shRNA plasmid was transfected into colorectal carcinoma SW480 cells line by liposome mediation for transient expression After Sp1 shRNA plasmid transfected SW480 cells the exogenous Sp1 protein expression was determined by the method of Western blot At same time hTERT mRNA expression was detected by RTPCR telomerase activity was determined by the telomeric repeat amplification protocol TRAP assay and the apoptotic rate of cells was also tested by flow cytometryThe protein expressions of Sp1 gene could be reduce by transfecting of pGenesil1Sp1+ recombinant plasmid into SW480 cells The apoptotic rate was increased compared with pGenesil1Sp1−/SW480 and SW480 P 005 which indicated that lowexpression of Sp1 gene could lead to low level of telomerase activity and induce apoptosis
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