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Title of Journal: Genet Resour Crop Evol

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Abbravation: Genetic Resources and Crop Evolution

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Kluwer Academic Publishers

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DOI

10.1002/lsm.1037

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1573-5109

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Genetic diversity of HMW glutenin subunit in Chine

Authors: Yong Liu ZhiYong Xiong YongGang He Peter R Shewry Guangyuan He
Publish Date: 2007/05/02
Volume: 54, Issue: 4, Pages: 865-874
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Abstract

The high molecular weight HMW glutenin subunit composition of 111 common landraces of bread wheat collected from Hubei province China has been determined by sodium dodecyl sulphate polyacrylamide gel electrophoresis SDSPAGE Ninety six of the accessions were homogeneous for HMW glutenin subunit composition and 15 were heterogeneous For the Glu1 loci 16 alleles were detected 3 at the GluA1locus 9 at the GluB1and 4 at the GluD1 Three novel alleles were identified two at the GluB1 and one at the GluD1locus Combination of these 16 alleles resulted in 14 different HMW subunit patterns The distribution of HMW glutenin subunit alleles in a subset of 105 of the 111 accessions representing six populations was assessed both at the individual population and whole population levels The results demonstrated that the distribution of allelic patterns varied among populations Taken together 625 of the alleles detected were considered to be rare alleles while the GluA1c null GluB1b 1Bx7 + 1By8 and GluD1a 1Dx2 + 1Dy12 alleles were found most frequently in the six populations The subset exhibited relatively high genetic diversity A = 533 P = 100 Ae = 1352 and He = 0238 with 815 of the diversity being within populations and 185 between populationsThis work was supported by grants from the Ministry of Science and Technology of China 2002CB111302 2002AA224031 and a grant from the National Natural Science Foundation of China 30370807 We thank Mr Qichang Zhang Institute of Crop Breeding and Cultivation Hubei Agricultural Academy of Science for providing the wheat landrace accessions


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