Journal Title
Title of Journal: Genet Resour Crop Evol
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Abbravation: Genetic Resources and Crop Evolution
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Publisher
Springer Netherlands
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Authors: Odireleng O Molosiwa Siise Aliyu Florian Stadler Katie Mayes Festo Massawe Andrzej Kilian Sean Mayes
Publish Date: 2015/02/26
Volume: 62, Issue: 8, Pages: 1225-1243
Abstract
We report here the development of 75 SSR markers for Bambara groundnut through various sequencing approaches and the subsequent utilization of these SSR markers and other marker types DArT Arrays for the analysis of genetic diversity population structure and the selection of pure lines from landraces To assess the level of genetic diversity in Bambara groundnut landrace collections 68 and 201 polymorphic SSR and DArT markers respectively were assayed against 24 single individual seed from a core collection of landraces Polymorphic information content PIC ranged from 008 to 089 with an average of 042 across all SSR polymorphic markers with observed Ho and expected He heterozygosity of 001 and 05 respectively For the population structure analysis 12 SSR markers selected as highly polymorphic from the initial screen were assayed against 123 landrace accessions The average PIC across the 12 SSR was 066 with an average Ho of 002 and an inbreeding coefficient f of 098 suggesting that seed derived from a single plant essentially represents an unselected variety Based on the low level of Ho and high f observed in the landrace samples a subset of 35 genotypes derived from the landraces were advanced for field trials in Botswana and scored for 37 phenotypic traits These 35 individual genotypes were ranked for agronomic performance based on yield component factors and other qualitative traits after field evaluation Five genotypes with good yield potential and adaptability were recommended as potential varieties for Botswanan agroecology
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