Multiple domestications of the Mesoamerican gene p

Authors: R H AnduezaNoh M L SerranoSerrano M I Chacón Sánchez I Sanchéz del Pino L CamachoPérez J CoelloCoello J Mijangos Cortes D G Debouck J MartínezCastillo

Publish Date: 2012/09/13

Volume: 60, Issue: 3, Pages: 1069-1086

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Abstract

The Mesoamerican MA gene pool of lima bean Phaseolus lunatus L is widely distributed from northern Mexico to northern Argentina in its wild forms and from the southern United States to the east coast of Brazil in its domesticated forms This broad distribution and lack of wild accessions of the MA gene pool in many areas of its natural distribution has impeded determining its center of domestication and establishing whether it has a single or multiple centers To answer these questions we evaluated 262 accessions of P lunatus using two intergenic spacers of chloroplast DNA atpBrbcL and trnLtrnF The data were analyzed using a maximum likelihood tree ML a haplotype network and two estimators of genetic differentiation N ST and G ST Nucleotide diversity π and haplotype Hd were quantified to estimate the percentage of reduction in genetic diversity r as a founder effect The ML tree and haplotype network indicated the existence of three groups AI MI and MII which was supported by the high values of N ST 061–080 Values for r were high 5867–6083  Existence of Mesoamerican and Andean gene pools was confirmed with two genetically and geographically distinct groups MI and MII within the MA gene pool We present the first evidence for multiple origins of domestication for the MA gene pool For MI we propose western central Mexico as the domestication area and between Guatemala and Costa Rica for MII We observed a founder effect in the MA gene pool as a result of domesticationThis paper is part of the first author’s Ph D Dissertation at the Centro de Investigación Científica de Yucatán A C postgraduate studies in Biological Sciences Option Natural Resources The research was conducted under the direction of Jaime MartínezCastillo The first author thanks the Consejo Nacional de Ciencia y TecnologíaMexico the scholarship for his postgraduate studies Ciencia BásicaCONACYT project number 82642 and National Geographic Society grant number 862209 for the economic support to carry out this research

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