Changes of gene expression in early generations of

Authors: Yong Zhuang JinFeng Chen

Publish Date: 2009/04/28

Volume: 56, Issue: 8, Pages: 1071-

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Abstract

Changes of gene expression played an important role in the evolution of plant allopolyploids Frequency time and type of the changes of gene expression between the first two selfpollinated generations S1 and S2 of a synthesized allotetraploid Cucumis × hytivus Chen et Kirkbride and in its diploid parents was analyzed with cDNAAFLP and reverseNorthern blot technique Sequences similarity of genes involved in changed expression were also analyzed with BLAST package The results from cDNAAFLP analysis showed that 36 337 genes showed silencing 27 or activation 9 in allotetraploids These changes initiated in S1 or S2 generation The silenced/activated genes included rRNA and proteincoding genes Further reverseNorthern blot analysis validated the results obtained Thus four types of changes of gene expression were observed including silencing of genes from both parents maternal parent paternal parent and genes novel expression The results indicate rapid changes in gene expression in early generations of C × hytivus which contributed to the evolution of this synthetic allotetraploidThis research was partially supported by the Key Program 30830079 and the General Program 30671419 and 30700541 from the National Natural Science Foundation of China the ‘863’ Programs 2008AA10Z150 2006AA10Z1A8 2006AA100108 the ‘973’ Program 2009CB119000 and the Ph D Funding 20050307009 from the Ministry of Education of the People’s Republic of China

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