Journal Title
Title of Journal: Genet Resour Crop Evol
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Abbravation: Genetic Resources and Crop Evolution
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Publisher
Springer Netherlands
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Authors: Michela Alfieri Laura Gazza Norberto E Pogna Rita Redaelli
Publish Date: 2014/05/28
Volume: 61, Issue: 8, Pages: 1481-1490
Abstract
Vromindolines a family of oat starchbound proteins responsible for the extremely soft endosperm of this cereal are characterized by a tryptophanrich domain and ten conserved cysteine residues as it was observed in the starchbound proteins from other cereals and proved to be specific of the genus Avena Twodimensional electrophoresis of vromindolines from different oat species both cultivated and wild revealed that they are present in all genomes and ploidy levels with no major differences in their molecular weight and electrophoretic mobility PCR amplification and sequencing of genes coding for VIN2 and VIN3 indicated that both proteins are strongly conserved across the species analysed Some differences were found between diploid and polyploid accessions at specific amino acid positions along the protein In particular the Cgenome accessions A clauda A eriantha A ventricosa differed from the Agenome diploids A canariensis A damascena A longiglumis A nuda A strigosa for some peculiar sequences Tetraploids A barbata A magna and A insularis showed two sequences each for both proteins the variability observed in them was similar to that found in A sativa The characteristics of these genes could help to clarify the genetic relationship among the various species of the genus Avena Moreover the knowledge of the genetic control of these proteins represent an important tool for the modulation of oat endosperm texture
Keywords:
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