Placental Alpha Hemoglobin Stabilizing Protein AH

Authors: Monica Emanuelli Monia Cecati Davide Sartini Piergiorgio Stortoni Alessandra Corradetti Stefano R Giannubilo Angelo Turi Andrea L Tranquilli

Publish Date: 2008/08/15

Volume: 14, Issue: 2, Pages: 193-197

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Abstract

AHSP inhibits cellular production of the reactive oxygen species Reduced AHSP indicates reduced protection against oxidative stressors Our objective was to investigate AHSP levels in recurrent miscarriage RM Trophoblast was collected from women of 10 weeks gestation voluntary abortion controls VA n = 10 spontaneous first miscarriage with subsequent normal pregnancy SMSN n = 15 or with subsequent miscarriage SMSM n = 5 RM previously investigated RMPS n = 5 or not previously investigated RM n = 5 AHSP mRNA and protein were determined using realtime quantitative polymerase chain reaction PCR and Western blot respectively Oneway ANOVA was performed to assess statistical significance p  005 ahsp mRNA levels were maximally reduced in RM and RMPS 80 × 10−6 ± 13 and 81 × 10−6 ± 07 respectively compared with SMSN and VA 161 × 10−6 ± 23 and 261 × 10−6 ± 27 respectively SMSM showed levels significantly reduced as well 90 × 10−6 ± 23 In RM a reduced defense from oxidative stressors is evident at first miscarriage identifying women at high risk for subsequent eventful pregnancy Reduced AHSP may identify women at risk of experiencing further miscarriages

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