Authors: Sabine M Schreier Markus K Muellner Hannes Steinkellner Marcela Hermann Harald Esterbauer Markus Exner Bernhard M K Gmeiner Stylianos Kapiotis Hilde Laggner
Publish Date: 2009/08/13
Volume: 17, Issue: 3, Pages: 249-256
Abstract
Highly reactive αβunsaturated aldehydes like 4hydroxy2nonenal 4HNE generated from oxidation of polyunsaturated fatty acids can bind to proteins polynucleotides and exert cytotoxicity 4HNE is known to react readily with thiol and amino groups on free or bound amino acids Recently hydrogen sulfide H2S has been identified as an endogenous vascular gasotransmitter and neuromodulator which can reach up to 160 μmol/l in the brain Markedly higher 4HNE concentrations were reported in the brain of patients suffering from Alzheimer’s disease Assuming that the low molecular thiol H2S may react with 4HNE we have tested the ability of H2S to counteract the cytotoxic and proteinmodifying activity of 4HNE The results show that H2S at physiologically relevant concentrations could effectively protect neuronal cells SHSY5Y from the cytotoxic action of 4HNE The HNEmodification of cellular proteins was also inhibited in presence of H2S These data suggest that H2S may be an important protective factor against carbonyl stress by inactivating/modulating the action of highly reactive αβunsaturated aldehydes like 4HNE in the brain
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