Journal Title
Title of Journal: Neurotox Res
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Abbravation: Neurotoxicity Research
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Publisher
Springer-Verlag
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Authors: Robson Luiz Puntel Daniel Henrique Roos Rodrigo Lopes Seeger Michael Aschner João Batista Teixeira Rocha
Publish Date: 2012/12/06
Volume: 24, Issue: 2, Pages: 109-118
Abstract
Organochalcogens such as organoselenium and organotellurium compounds can be neurotoxic to rodents Since mitochondrial dysfunction plays a pivotal role in neurological disorders the present study was designed to test the hypothesis that rat brain mitochondrial complexes I II I–III II–III and IV could be molecular targets of organochalcogens The results show that organochalcogens caused statistically significant inhibition of mitochondrial complex I activity which was prevented by preincubation with NADH and fully blunted by reduced glutathione GSH Mitochondrial complex II activity remained unchanged in response to PhSe2 treatment Ebs and PhTe2 caused a significant concentrationdependent inhibition of complex II that was also blunted by GSH Mitochondrial complex IV activity was not modified by organochalcogens Collectively Ebs PhSe2 and PhTe2 were more effective inhibitors of brain mitochondrial complex I than of complex II whereas they did not affect complex IV These observations are consistent with organochalcogens inducing mitochondrial complex I and II inhibition via their thioloxidaselike activity with Ebs PhSe2 and PhTe2 effectively oxidising critical thiol groups of these complexesThis work was supported by grants from UNIPAMPA Universidade Federal do Pampa UFSM Universidade Federal de Santa Maria FAPERGS Fundação de Amparo a Pesquisa do Estado do Rio Grande do Sul CAPES Coordenação de Aperfeiçoamento de Pessoal de Nível Superior CNPq Conselho Nacional de Desenvolvimento Científico e Tecnológico FINEP Rede Instituto Brasileiro de Neurociência IBNNet 0106084200 and INCTEN Instituto Nacional de Ciência e Tecnologia em Excitotoxicidade e Neuroproteção MA was supported in part by Grants from the National Institutes of Health ES R01 07331 ES R011 0563 and ES P30 000267
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