Journal Title
Title of Journal: Neurotox Res
|
Abbravation: Neurotoxicity Research
|
Publisher
Springer-Verlag
|
|
|
|
|
|
Authors: Fernanda Martins Lopes Giovana Ferreira Londero Liana Marengo de Medeiros Leonardo Lisbôa da Motta Guilherme Antônio Behr Valeska Aguiar de Oliveira Mohammad Ibrahim José Cláudio Fonseca Moreira Lisiane de Oliveira Porciúncula João Batista Teixeira da Rocha Fábio Klamt
Publish Date: 2012/01/20
Volume: 22, Issue: 2, Pages: 138-149
Abstract
It is well established that oxidative stress plays a major role in several neurodegenerative conditions like Parkinson disease PD Hence there is an enormous effort for the development of new antioxidants compounds with therapeutic potential for the management of PD such as synthetic organoselenides molecules In this study we selected between nine different synthetic organoselenides the most eligible ones for further neuroprotection assays using the differentiated human neuroblastoma SHSY5Y cell line as in vitro model Neuronal differentiation of exponentially growing human neuroblastoma SHSY5Y cells was triggered by cultivating cells with DMEM/F12 medium with 1 of fetal bovine serum FBS with the combination of 10 μM retinoic acid for 7 days Differentiated cells were further incubated with different concentrations of nine organoselenides 01 03 3 10 and 30 μM for 24 h and cell viability neurites densities and the immunocontent of neuronal markers were evaluated Peroxyl radical scavenging potential of each compound was determined with TRAP assay Three organoselenides tested presented low cytotoxicity and high antioxidant properties Pretreatment of cells with those compounds for 24 h lead to a significantly neuroprotection against 6hydroxydopamine 6OHDA toxicity which were directly related to their antioxidant properties Neuroprotective activity of all three organoselenides was compared to diphenyl diselenide PhSe2 the simplest of the diaryl diselenides tested Our results demonstrate that differentiated human SHSY5Y cells are suitable cellular model to evaluate neuroprotective/neurotoxic role of compounds and support further evaluation of selected organoselenium molecules as potential pharmacological and therapeutic drugs in the treatment of PD
Keywords:
.
|
Other Papers In This Journal:
|