Authors: Yaqin Xu Lizanne Pharand Quan Wen Ferdinand Gonzaga Yingfu Li M Monsur Ali Carlos D M Filipe Robert Pelton
Publish Date: 2010/11/02
Volume: 289, Issue: 5-6, Pages: 659-666
Abstract
Compared are two approaches for the biotinylation of polyNisopropylacrylamidecovinylacetic acid microgels 300nm diameter water swollen particles with a corona of carboxyl groups The biotinylated microgels are a platform for bioactive waterbased ink Streptavidin binding was measured as a function of biotin density and the results were interpreted with a new model that predicts the minimum local density of biotins required to capture a streptavidin An aminopolyethylene glycol derivative of biotin gave higher biotin contents than a biotin hydrazide However the streptavidin content versus biotin content results for both biotin derivatives fell on the same master curve with maximum biotin coverage of 011 mg of bound streptavidin per milligram of biotinylated microgel Exclusion experiments showed that streptavidin was too big to penetrate the crosslinked microgel structure thus the conjugated streptavidin was restricted to the microgel surface The colloidal stability of the microgels was preserved and the biotinylated products showed good hydrolytic stability
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