Journal Title
Title of Journal: Trop Anim Health Prod
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Abbravation: Tropical Animal Health and Production
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Publisher
Springer Netherlands
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Authors: Gholamreza Moazeni Jula Morteza Sattari Reza Banihashemi Hossein Razzaz Alireza Sanchouli Keyvan Tadayon
Publish Date: 2010/11/30
Volume: 43, Issue: 3, Pages: 699-704
Abstract
To understand epidemiology of Bacillus anthracis in Iran the morphological biochemical and virulence specifications of 32 B anthracis isolates collected from human sheep cattle goat and environmental specimens obtained from throughout Iran were examined by conventional and molecular approaches B anthracis isolates were characterized in multiple ways 1 capsule formation both on bicarbonate agar and in defibrinated horse blood 2 motility of vegetative forms 3 hemolysis on 5 sheep blood agar 4 penicillin G susceptibility 5 lecithinase production on egg yolk agar 6 gelatin hydrolysis 7 ability to develop “string of pearls” on tryptose agar and 8 capability to develop mucoid colonies in presence of CO2 were assessed In addition biochemical properties such as indole methyl red catalase citrate utilization and finally nitrate reduction tests were used All the tested isolates produced identical morphological and biochemical patterns with those of the vaccine strain B anthracis 34F2 Sterne In order to assess potential virulence of isolates at genomic level PCR protocols assaying for the pXO1 and pXO2 loci were employed The intriguing high level of phenotypic similarity between Iranian isolates of B anthracis and the 34F2 Sterne strain deserves further studies at genomic levelWe gratefully acknowledge the regional technical staff of the Iranian Veterinary Organization IVO for their assistance in provision of animal samples All staff at the Aerobic Bacterial Research and Vaccine Production Department of Razi Institute are thanked for their contribution to conduction of this research Dr Paul Keim is much thanked for critically reading and excellent editing the manuscript Morteza Sattari a highly respected academician at the Tarbiat Modares University sadly passed away in 2010 He will be missed by all his colleagues and friends
Keywords:
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- Improvement of trivalent leptospira vaccine by removal of anaphylactic agents
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- Molecular detection of pestiviruses in aborted foetuses from provinces in northern Turkey
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